Road to the Healthier Pet

What is DOG-EGF?

Discovery of EGF (Epidermal Growth Factor)

leads up to DOG-EGF

Dr. Stanley Cohen of the United States, who studied the mechanism of skin aging, discovered that senescence occurs due to a decrease in EGF (Epidermal Growth Factor.) He was given the Nobel Prize in physiology in 1986 for recognition for his achievement.

* What is EGF? It is an epidermal cell growth factor protein that helps skin cell growth and production and restores the cell's original self healing power.

World's first!

Registration of DOG-EGF in International Cosmetic Ingredients (ICID)

New concept of bio-regeneration care for pets: DOG-EGF

Mumutto's DOG-EGF (sr-dog polypeptide-1) was registered in International Cosmetic Ingredient (ICID) for the first time ever. It is an EGF of a pet. (※ Date of registration : March 11, 2016)

This pet skin peptide not only improves the immunity of the coat but also regenerates damaged skin, and is effective for various skin problems.

My Pet's Health Practitioner

 

EGF is an epidermal cell growth factor present in the skin.

(It is a form of protein that helps the growth and

reproduction of skin cells, leading to the healthy skin.)

Over time, EGF decreases, causing skin aging.

It is very important to protect the pet's skin by maintaining DOG-EGF.

Mumutto discovered EGF in pets and further adapted and developed DOG-EGF (sr-dog polypeptide-1.)

In addition, it is the first to register DOG-EGF (sr-dog polypeptide-1) on International Cosmetic Ingredients (ICID).

(※ registration date: March 11, 2016)

※ DOG-EGF is the product name of raw materials developed by us.

Development of DOG-EGF

※ DOG-EGF promotes proliferation and reproduction of skin cells with EGF of pets, and strengthens the skin barrier with protein.

※ We thoroughly and safely cultivated with a ISO 9001 certified company.

DOG-EGF's Genetic code

We discovered DOG-EGF, which is safe and secure under any circumstances.

Figure 1. Synthesis of DOG-EGF (sr-dog polypeptide-1) through Nucleotide Sequence Analysis

Figure 2. High purity DOG-EGF (sr-dog polypeptide-1) found in suitable temperature and conditions

DOG-EGF gene synthesis clone preparation

DOG-EGF purification technology development

DOG-EGF purification technology development

DOG-EGF purification technology development

DOG-EGF gene synthesis clone preparation

Comparison of DOG-EGF's Effect on Cell Proliferation

BEFORE

BEFORE

AFTER

AFTER

A: human epidermal keratinocyte cell line (HaCaT) - non-treated group

DOG-EGF 1 ppm 24 hrs after application

B: Canine kidney epithelial cell line: MDCK (NBL-2) - untreated group

DOG-EGF 1 ppm 24 hrs after application

In order to investigate the degree of cell proliferation of DOG-EGF,

DOG-EGF (1 ppm) was applied to A and B and observation

Condition A (human epidermal keratinocyte cell line: HaCaT): cell proliferation observatio

Condition B (canine kidney epithelial cell line: MDCK (NBL-2)): active cell proliferation observed

Comparison Experiment of DOG-EGF Activity Rate

<Human Epidermal Keratinocyte Cell Line: HaCaT>

Activity Rate A(U/ml) = 9*10

5

<Canine Kidney Epidermal Cell Line: MDCK (NBL-2)>

Activity Rate A(U/ml) = 5.6*10

6

Activity rate data analysis result of DOG-EGF,
Human epidermal keratinocytes cell line (HaCaT) also showed active reponse,

About 6 times higher activity was measured in canine kidney epidermal cell line (MDCK (NBL - 2)).

Bio-Regeneration Care DOG-EGF (sr-dog polypeptide-1)

■ World's First International Cosmetic Ingredients (ICID) Registration

    It was the first in the world to register

    in International Cosmetic Ingredients Raw Material (ICID).

■ DOG-EGF activates the EGF originally present in the pet's body

    It improves troubles such as skin / coat troubles,

    and leads to healthy skin and coat.

■ Regeneration care for making durable skin

    For pets suffering from atopy, the skin recovery care restores skin

    to health without other stimulation.
 

■ Boosts cell regeneration and collagen production

    By combining bioscience and biotechnology,

    it promotes proper cell growth and collagen production in dog's skin cells.

MUMUTTO

SNS

KN Global Corporation

Phoenix Higashiginza 305, 4-2-7, Tsukiji, Chuo-ku, Tokyo, Japan